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To assess the functional integrity of the isolated mitochondria, we measured the changes in mitochondrial membrane potential (MMP, m) using the 5,5,6,6-tetrachloro-1,1,3,3-tetraethyl benzimidazolyl carbocyanine iodide (JC-1) probe (1st J-aggregate-forming cationic dye) as previously described 60

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Likewise, such neuroinflammation can interact with neurotransmitters, elevate procoagulant activity and thrombosis, and cause endothelial damage, resulting in neurovascular coupling (NVC) dysfunction (363)