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Description
Agonist dissociation was monitored immediately after washing in 10 M exendin(9-39) by TR-FRET at 37 C, with 50 s delay and 300 s integration time, and binding quantified as 520 nm over 620 nm signal after nonspecific binding subtraction with excess (10 M) unlabeled agonist
155 However, whether this can be extrapolated to other CMRFs, and whether there is a pharmacoeconomic imperative, needs further clarification
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Gunturu S
