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Site-specific pegylated Exendin-4 modified with a high molecular weight trimeric PEG reduces steric hindrance and increases type 2 antidiabetic therapeutic effects

Additionally, dissociated G subunits can also trigger signalling that leads to activation of both the calcium and the extracellular signal-regulated kinase (ERK1/2) pathways (Lymperopoulos et al., 2022)

The complex was subjected to size-exclusion chromatography (SEC) on a Superose 6 Increase 10/300 column (GE Healthcare) equilibrated with 20 mM HEPES pH 7.5, 100 mM NaCl, 2 mM MgCl 2 , 1 M semaglutide, 0.01% (w/v) LMNG, and 0.006% (w/v) CHS

Proc Natl Acad Sci U S A (2003) 100(9):50638