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This suggests that there may be significant age-dependent neurotransmission differences in the D1-D2 receptor heteromeric pathway combined with an in situ proximity ligation assay (PLA) technique with different neuronal markers to characterize the neurons expressing D1-D2 receptor heterodimers in the striatum (including the caudate nucleus, the putamen, and the NAc core and shell of the), finding heterodimers in all striatal regions and projection neurons of the direct and indirect basal ganglia pathways (Rico et al., 2017)