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This paradigm shifted with the advent of high-throughput sequencing technologies, particularly 16S rRNA gene profiling and shotgun metagenomics, which unveiled a complex ecosystem harboring several hundred to over a thousand microbial species across the nasopharynx to alveoli [89]

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Wright LN, Thorp JM Jr, Kuller JA, Shrewsbury RP, Ananth C, Hartmann K
