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RNA extraction, reverse-transcription PCR (RT-PCR), and reverse transcription quantitative real-time PCR (qRT-PCR) The inoculated leaves were harvested at 0-, 1-, 3-, and 9 days post inoculation (dpi) for the determination of virus accumulation and gene expression

Adult perceptions of the relative harm of tobacco products and subsequent tobacco product use: longitudinal findings from waves 1 and 2 of the population assessment of tobacco and health (path) study