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A.CatalanoM.MiglioreR.MiglioreM.et al

The different stratified skin layers were suspended in methanol and the copper peptides were extracted by ultrasonication

Collected pellets (containing PLGA nanoparticles) and supernatant (containing leached AuNCs as they do not sediment at this centrifugation conditions) were analyzed using ICP-MS
Each sample was gently rinsed twice with 20 l double-deionized water
