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Targeted demethylation of specific loci successfully restored sperm count by 80%, confirming that DNA methylation changes represent functional rather than merely correlative alterations ( 5.4.2 Single-cell epigenomic profiling Advanced single-cell technologies including scRNA-seq and ATAC-seq have revealed persistent chromatin accessibility changes in F1-F3 germ cells, providing unprecedented resolution of transgenerational epigenetic inheritance mechanisms ( The convergence of evidence from animal models, human cohort studies, and mechanistic investigations strongly supports the conclusion that EED exposure creates heritable reproductive dysfunction through epigenetic mechanisms, fundamentally expanding our understanding of environmental health risks beyond individual exposure effects