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2-AB metabolism in vitro and in vivo For in vitro synthesis of 2-AB, 2-oxobutyric acid was mixed with 50 mM Tris-HCl, 0.1 mM pyridoxal-5-phosphate, 0.1 mM glutamic acid, and 5 units of aspartate aminotransferase (WAKO)

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(2007) identified a cell population with trilineage differentiation capacity in the human annulus fibrosus
