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After introducing gfa, flhA and fghA into BL21 and screening the second generation of formaldehydetic strain (FDS-2) through colony PCR and SDS-PAGE electrophoresis, we found that the formaldehydetic tolerance of FDS-2 was improved to some extent compared with that of BL21 through the determination of its formaldehyde tolerance, enzyme activity, the degradation rate of formaldehyde and the maximum amount of formaldehyde that can be degraded