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A 10 min gradient elution program was applied for target compounds separation with 0.1% formic acid in acetonitrile (A) and pure water (B) as mobile phase with a 200 L/min flow rate

Social media pressure and physique culture : The rise of Instagram and TikTok fitness influencers has elevated lean, muscular physiques as the gold standard

NF- B, nuclear factor kappa-light-chain-enhancer of activated B

The primary antibodies that were used were as follows: antibodies against AMPK (#2532, 1: 1000), phospho-AMPK (#2535, 1: 1000), and -smooth muscle actin (#19245 T, 1: 1000) were obtained from Cell Signaling Technology (MA, USA)