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A second enzyme that cleaves -glutamyl bonds (converting the glutathione S -conjugate LTC 4 to the corresponding cysteinyl-glycine conjugate LTD 4 ) was identified independently by two groups in the 1990s and named GGT-rel and -glutamyl leukotrienase (Carter et al., 1997, Heisterkamp et al., 1991)

Usually one of the these strategies will work but in case they still do not work, then one can go for use of larger affinity tag such as GST or MBP instead of small Histidine tag.

[DOI] [PubMed] [Google Scholar] 26.Xu Z.N., Lin Z.Q., Zhao G.S., Guo Y.B

In seiner aktiven Form wird es GSH genannt, wobei SH die Schwefel-Wasserstoffgruppe bzw