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reported that calcium deficiency leads to male infertility by activating various mechanisms, such as spermatogenesis failure, impaired steroidogenesis, chemotactic failure, and impaired sperm motility, capacitation, and acrosome reaction (110)
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A 498 bp DIG labeled probe, which hybridizes 5 upstream of the replacement vector, was generated by PCR using 5 forward primer CTTACAGCATGGGCAGCAGACTC and 5 reverse primer GTCTCCTCCACGGGGGTCAG and Roche labeling kit (catalog 11636090910)
