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A topical numbing cream is applied before the procedure
In vitro techniques constitute an effective alternate approach simulating in vivo animal models, as the latter approach is reasonably more costly and raises serious ethical issues [18,19,20]

Dickerhof N, Pearson JF, Hoskin TS et al (2017) Oxidative stress in early cystic fibrosis lung disease is exacerbated by airway glutathione deficiency
Brain 125:15221533 Dal Canto MC, Gurney ME (1995) Neuropathological changes in two lines of mice carrying a transgene for mutant human Cu,Zn SOD, and in mice overexpressing wild type human SOD: a model of familial amyotrophic lateral sclerosis (FALS)
