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volume/volume (v/v)) was added to the homogenates and vigorously vortexed for 1 minute followed by shaking at 4 C at 1200 RPM for 30 minutes using a thermomixer (Eppendorf, Hamburg, Germany)
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It has been shown that the NTD of hNPC1 binds cholesterol and 25-hydroxycholesterol tightly (35)
This means it is needed to preserve muscle tissue during dieting and exercise
