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Here, we describe the GST-His method as a new small-scale affinity purification system for recombinant proteins, based on a N-terminal Glutathione Sepharose Tag (GST) 2,3 and a C-terminal 10xHis tag 4 , which are both fused to the protein of interest

Apple in Bladder Cancer Prevention The antioxidant and anti-inflammatory properties of the compounds present in apples may also help reduce BCs development

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With a combination of methods drawn from classical genetics, the state-of-the-art in quantitative imaging, and new approaches to manipulating single cells in vivo, we are mapping the emergence of functional circuitry in the embryo