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Whole eyes were extracted, fixed in 4% PFA for 30 min, and then cryoprotected in 30% sucrose with 0.02% sodium azide overnight

doi: 10.1016/0022-2828(95)90073-x

doi: 10.1002/pca.3411 100 BambaT

First, direct pH modifications alter the environment of the digestive tract