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The resulting supernatant was then transferred to new tubes and stored in a 80C freezer

American Family Physician 2006;73:65

Don't guess or estimate your dose

To address this issue, researchers must focus on optimizing methods that account for stemness stability through modulating signaling pathways, such as Notch and Wnt/-catenin [135], or using small molecules to enhance SCs self-renewal capabilities, including glutathione, melatonin, and ascorbic acid (ASA) [136]
