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Toscano, F

These tests may require specialist laboratory referral Holotranscobalamin (active B12) where available, may be used as a second-line test when standard B12 results are borderline Intrinsic factor antibodies to identify pernicious anaemia, the most common cause in the UK Parietal cell antibodies may be useful if intrinsic factor antibodies are negative but pernicious anaemia is still suspected Thyroid function and folate levels to exclude other causes of similar symptoms According to current UK guidance, patients presenting with neurological symptoms suggestive of B12 deficiency should not have treatment delayed whilst awaiting test results

The resulting fluorescence patterns closely resembled those observed with the redox-sensitive H2B-Grx1-roGFP2 sensor, suggesting that the ratio increase during mitosis is not related to changes in E GSH and thus, it likely resulted from focus adjustments due to cell detachment and rounding during division

EPR spectroscopy allowed recognizing the generation of reaction products of DMPO that derive from its reaction with superoxide (in its turn, recognized from its disappearance from the reaction mixture after incubation with superoxide dismutase)