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The pcDNA3.1/ myc -His()A-( G 4 C 2 ) n vector was digested with EcoR I and Xba I, and subcloned into the Drosophila pUAST vector

The reason for this is that the initial wave of drug-induced hepatocellular destruction is followed by a robust innate immune response, in which invading inflammatory cells release toxic oxidants and cause a second wave of destruction

This gentle addition prevents foaming and possible peptide breakdown

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