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This topic is another analytical challenge by itself, especially since the quantification process requires not only establishing the whole content of the peptide in the sample (using, e.g., amino acid analysis, with TLC, HPLC, GC-MS, LCMS, CE, or RMN methods) but also the content of the counterion (using either LC or GC methodologies), water/humidity content (using Karl Fischer), and impurities tests for the evaluation of residual solvents (such as ACN, DMF, DCM through GC-HS-MS), heavy metals, and peptide-related and nonrelated impurities

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