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Experimental approachesinitial screening of four drug candidates against SARS-CoV-2 CaCo-2 and Calu-3 cells were seeded into a 96-well flat bottom plate with 1 104 (CaCo-2) or 4 104 (Calu-3) cells per well
prolifera extraction using cellulase (Novozymes Co., Ltd., Copenhagen, Denmark), the enzyme amount was 2% (w/v), the ratio of algae to water was 1:30 (w/v), and the extraction was performed at 50C for 1.5 h at pH 5.0
doi: 10.1128/AAC.00684-06 76 LeP
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