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Acetyl-CoA serves as a pivotal central metabolite and precursor in the intricate fatty acid synthesis process [75]

1.3.1 Cytoplasmic hybrid (cybrid) cell lines First derived in 1975 by Wallace, Bunn, and Eisenstadt, cytoplasmic hybrid (cybrid) cell lines are generated by the fusing of nucleated cells that have had their endogenous mtDNA depleted via exposure to ethidium bromide with enucleated cells (cytoplasts), which harbor the mtDNA signature of interest (Figure 3A