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Furthermore, relevant studies have demonstrated the diagnostic value of USWE in visualizing myofascial trigger points and spasticity, supporting the clinical use of ultrasound-guided therapies for pain modulation 48,49,50

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Intestinal microbial dysbiosis completely affects immunomodulatory networks, is strongly associated with immune-mediated diseases and gut inflammation (Maynard et al., 2012) and has been proven experimentally in pearl gentian grouper fish (Deng et al., 2020) and sea cucumbers (Zhao et al., 2019)

Notably, hypoxic conditions within the TME further amplify glycolytic flux through hypoxia-inducible factor 1 (HIF-1)mediated transcriptional activation of glycolysis-related genes, thereby establishing a metabolic landscape that favors HCC survival and progression [35,36,43,44] [Figure 1]