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Such cultures have been described previously, in which intestinal cells were cultured on a monolayer of myofibroblasts 39

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It was suggested that PX-478 lowers HIF-1 levels by affecting multiple levels in the HIF-1 pathway: (1) inhibition of HIF-1 deubiquitination via p53 and pVHL independent mechanism, resulting in increased levels of polyubiquitinated HIF-1

Defects in formation of FANCD2 nuclear foci after cisplatin treatment can be used to indicate cisplatin sensitivity [17] and concomitant overexpression of PARP1 , FANCD2 , and TP53 predicted increasing resistance to platinum [18]