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Description
50 Using serum metabolomics data available in a subset of participants (n = 154 pre-menopausal and n = 192 post-menopausal), we observed that gut species and functions depleted in menopause, including [Clostridium] lactatifermentans, Akkermansia muciniphila , and the -glucuronidase gene ortholog, were positively correlated with serum progestin metabolites in post-menopausal women, but not in pre-menopausal women

We recommend starting with the same daily schedule for three months, until symptoms stop improving, and then starting to test

Generation of Mpst knockout (KO) mice We generated Mpst deficient mice by genome editing using the CRISPR/Cas9 nickase in the genetic background of the C3H strain (Appendix Fig S6)

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