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doi: 10.1007/s40256-019-00384-y

De Lange ECM, Vd Berg DJ, Bellanti F, Voskuyl RA, Syvanen S

Then, 20 L of MTT solutions was added to each well followed by an incubation period of 3 hours, then the media were removed and 200 l of DMSO was added to dissolve the crystal and the absorbance was recorded at 570 nm (BioTek, USA) and cell viability was calculated as a percentage compared to cells treated with DMEM alone

Indeed, patients with severely compromised GSH levels die early in life, usually before birth (Njalsson and Norgren, 2005
