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Non-esterified short-chain fatty acid (SCFA) quantification After finding that nonesterified short-chain fatty acids (SCFAs) were not detected by our first approach, we measured acetic, propanoic, isobutanoic (isobutyric), butanoic (butyric), isopentanoic (isovaleric), pentanoic (valeric), hexanoic (caproic) and heptanoic acids with a Clarus 500 chromatography system connected to a SQ8s mass spectrometer (Perkin Elmer) using the method described by Togo et al 50

BPC157 solution for administration was prepared by diluting the required amount of concentrated BPC157 solution in 0.9% NaCl injection solution prior to administration

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Furthermore, only four studies reported MCID thresholds, and none performed responder analyses beyond the 50% VAS reduction criterion (Shokri et al., 2021