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In the fatty acid oxidation (FAO) assay, the culture medium was washed twice and replaced with substrate-limited DMEM (102353-100, Seahorse Bioscience, Billerica, MA, USA) supplemented with 25 mM d-glucose, 1 mM sodium pyruvate, 0.5 mM l-carnitine hydrochloride (07353-51, Nacalai Tesque, Kyoto, Japan) and 0.5 mM NaOH to adjust to pH 7.4, and the cells were equilibrated for 1 h at 33 C in an incubator (0.03% CO 2 ) prior to the assay

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Nitric oxide helps blood vessels relax, which supports vascular tone, blood flow and healthy blood pressure