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10.1158/0008-5472.can-03-3294 110 McMahonM.ItohK.YamamotoM.HayesJ
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Co-immunoprecipitation To examine protein interactions, cell lysates were prepared from HEK-293T cells transiently co-transfected with epitope-tagged protein expression vectors (Flag-EV, IR WT-Flag, IR P963V -Flag, IR S968V -Flag, IR L973F -Flag, IR S976A -Flag, IR Y972F -Flag, HA-EV, Shc-HA as indicated)
The membrane is much more difficult to rearrange than intracellular metabolic pathways
