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Description
Hypothalamic regulation of the pituitary hormones
USA 114 , E2357E2364 (2017)

The design principle: Standard (often fails): His6 - TEV site - Target protein Better: His6 - TEV site - (GGS)2 - Target protein Even better: His6 - (GGS)2 - TEV site - (GGS)2 - Target protein Failure 2: Non-Specific Cleavage (The Protease Damages Your Protein) The problem: Proteases aren't perfectly specific

Proteomic responses to oxidative damage in meat from ducks exposed to heat stress