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Sample analysis was performed on an Agilent 1290 UPLC system equipped with a Waters BEH amide (2.1 mm 100 mm, 1.7 m) column using a gradient of 15 mM NHAc/0.3% NHOH in HO (A) and 90% ACN (B) at 0.3 mL/min

Due to multiple probe sets mapping to the same gene, the fold changes in m-RNA production produced by GenePattern were converted to percentages, and then all probe sets representing the same gene were averaged

Gradual progression respecting body signals