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The aim is to minimize confounding factors and obtain cleaner biological signals

Relative expression was determined by the 2 Ct method using the housekeeping gene, GAPDH, as an internal control, and the fold change was calculated by its comparison with the corresponding control group [28]

doi: 10.1111/j.1365-2125.2007.02990.x
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Shankar E, Goel A, Gupta K, Gupta S
