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CHF6001 II: a novel phosphodiesterase 4 inhibitor, suitable for topical pulmonary administration-in vivo preclinical pharmacology profile defines a potent anti-inflammatory compound with a wide therapeutic window

dNTPs (1 M of dATP, dGTP, dCTP, 025 M dTTP and 0.25 M aminoallyl-dUTP-ATTO-647N) were incorporated at the damage sites in 1 NEBuffer 2 using DNA polymerase I (1.25 U) at 20 C

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