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Description
Restriction of amino acids extends lifespan in Drosophila melanogaster

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For protein purification, harvested cells were resuspended with ice-cold lysis buffer (50 mM Tris-Cl pH 8.0, 300 mM NaCl, and cOmplete, EDTA-free protease inhibitor cocktail tablets (Roche)) at a ratio of 3.2 mL/g of cell pellet

Mistake #6: Forgetting to Hydrate from Within The problem: Perfect routine but not drinking enough water The fix: Aim for 8 glasses of water daily
