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For fixation, an equal volume of PBS (Thermo Fisher Scientific) + 8% paraformaldehyde (Electron Microscopy Sciences) was added to the medium for 15 min, after which the samples were washed three times with PBS

Thyroid hormone-regulated mouse cerebral cortex genes are differentially dependent on the source of the hormone: a study in monocarboxylate transporter-8- and deiodinase-2-deficient mice

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Common mistakes researchers make with pineal peptides The difference between researchers who report significant sleep improvements and those who see minimal results often comes down to protocol errors rather than peptide quality