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This activation is triggered by various stimuli, such as free radical scavengers, hypoxic insults, ionizing electromagnetic radiation, chemical substances, heat shock, and heavy metals [86, 87]
In particular, Cao and colleagues conducted a prospective cohort study (NCT00835341) to evaluate the predictive value of p16 methylation in patients diagnosed with oral epithelial dysplasia, demonstrating that the progression rates to OC were significantly higher in patients with p16 promoter hypermethylation compared to those with unmethylated p16 [278]
Meng et al., 2022
Where indicated, cells were washed in PBS, and re-plated in fresh medium in the absence of curcumin and imatinib
