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Dysregulation of pyroptosis can lead to diseases such as sepsis, autoimmune diseases, and neuroinflammation

Reactive oxygen species, primarily H 2 O 2, generation, was measured in HCM (7,500 cells per well, 10 M DOX, 4.4 M, B-017/negative control peptide, 4 h), HCF (80,000 cells per well, 500 nM STS, 52 M B-017, 1 h), HEK293 (80,000 cells/ per well, 500 nM STS, 52 M B-017, 1 h) and MCF-7 cells (80,000 cells per well, 500 nM STS, 52 M B-017, 1 h) using the DCFDA/H 2 DCFDACellular ROS Assay Kit (Abcam, ab113851), according to the manufacturers instructions

The interval between diapedesis and death may be too short for reactive changes, visible by light microscopy, to develop in the vessels