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At day 35 of differentiation, the amplitude of the burst fired by mOrange neurons was approximately 12% larger than those fired by mOrange + neurons (2.954 0.127 vs 3.296 0.050

After fatty acid treatment, the organoids were incubated with 3.8 M BODIPY 493/503 (Life Technologies) for 30 min in culture medium at 37 C and then washed two times with PBS before being replaced with fresh culture medium containing DRAQ5 (Thermo Fisher) at 1:1500

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The mechanistic classification of chronic pain into nociceptive, neuropathic, and nociplastic subtypes provides an essential framework for moving beyond symptomatic management toward pathophysiologydriven therapeutic strategies