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in its active form (thiamine diphosphate), it is an essential cofactor for enzymes in glucose and amino acid catabolism, helping convert nutrients into ATP and supporting normal neuronal function

Recordings were obtained using a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA), Analog signals were low-pass Bessel filtered at 2 kHz, digitized at 10 kHz through a Digidata 144 OA interface (Molecular Devices), and stored in a computer using Clampex 10.2 software (Molecular Devices)

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