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in its active form (thiamine diphosphate), it is an essential cofactor for enzymes in glucose and amino acid catabolism, helping convert nutrients into ATP and supporting normal neuronal function

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Recordings were obtained using a Multiclamp 700B amplifier (Molecular Devices, Sunnyvale, CA), Analog signals were low-pass Bessel filtered at 2 kHz, digitized at 10 kHz through a Digidata 144 OA interface (Molecular Devices), and stored in a computer using Clampex 10.2 software (Molecular Devices)

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