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Finally, after another wash, cells were incubated at RT for 30 min with a cocktail of phenotyping antibodies including CD3 BV510 (BD Biosciences Cat# 740187, Lot# 0261796), CD4 BV510 (BD Biosciences Cat# 562970, Lot# 0177290), CD14 BV510 (BioLegend Cat# 301842, Lot# B264335), and CD16 BV510 (Biosciences Cat# 563830, Lot# 1307468) as dump channel markers, and CD19 PE-Cy5 (Beckman Coulter Cat# IM2643U Lot# 200100), IgG PE-Cy7 (BioLegend Cat# 410721 Lot# M1310G05), IgM BV650 (Biolegned Cat# 314525 Lot# B294373)