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Description
Consistent use often leads to faster effects
Cells were lysed in 100 L ice cold 8 M Urea/50 mM Tris HCl lysis buffer (Fisher Scientific) with 1:100 of Halt Cocktail Inhibitor, EDTA-free (100X) (Thermo Scientific)
After this phase, we usually shift to a maintenance schedule of one injection every one to three months
NAD+ metabolism and its roles in cellular processes during ageing
