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Whole blood and plasma samples of six JVC rats were collected at 0.05, 0.167, 0.5, 1, 2, 4, 8, 24, 48, and 72 h after administration (three males and three females at each time point) for the examination of radio pharmacokinetics of total plasma

A 500mcg daily protocol under split dosing would look like: 300mcg upon waking (fasted), then 200mcg approximately 30 minutes before dinner or before bed on an empty stomach