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As many of these studies have used different strains, sexes and ages of mice, it has been difficult to interpret results between experiments, highlighting the need for a more standardized procedure (Zirngibl et al., 2022)

Dark areas on the knuckles or all over hyperpigmentation of the body may be seen

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Stifano G, Affandi AJ, Mathes AL, Rice LM, Nakerakanti S, Nazari B, Lee J, Christmann RB, Lafyatis R (2014) Chronic Toll-like receptor 4 stimulation in skin induces inflammation, macrophage activation, transforming growth factor beta signature gene expression, and fibrosis
