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After an overnight incubation at 4 C with primary antibodies against FAT/CD36 and CPT (Santa Cruz Biotechnology, USA), the membranes were washed with PBS-T and incubated with an HRP-conjugated secondary antibody for 1 h at 25 C
2020;219:e202003131
Mapping alterations of lipid C=C isomers on tissue sections by MELDI-DESI MSI techniques allow visualization of compounds in biological tissue sections, providing chemical-rich information associated with tissue heterogeneity
The pathway activates PI3K/Akt/Nrf2 signaling in neural tissue
