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Using simultaneous principal curves and orthogonal projection, Slingshot subsequently fits each differentiation lineage on a two-dimensional space and calculates the corresponding pseudotime values for the cell clusters, resulting in multiple linear cell differentiation trajectories originating from a common starting cell cluster
doi: 10.1038/oby.2010.54 23
Oxidative influence in development and differentiation: an overview of a free radical theory of development
To test this possibility, we co-immunostained glucagon and the Golgi marker GM130 in TC1-6 cells
