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In addition, glutamine starvation triggers the impairment of the cystine/glutamate antiporter system and decreases glutathione levels in the cell [13]

Nutraceuticals, sometimes referred to as dietary supplements, contribute health benefits, including disease prevention, however, they are considered nonpharmaceutical or nonmedicinal

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To construct the shRNA knockdown vector pLKO.1-GST DAF , we inserted the GST-EGFP-GPI DAF coding region into the pLKO.1 puro plasmid in place of the puromycin resistance gene