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Following the addition of 6 ml acetone containing BHT (0.1 %, w/v), the homogenates were thoroughly mixed using a Ohaus Multi-Tube Vortex mixer, for 5 min at 2,500 rpm, allowed to stand for 5 min, and centrifuged at 2,200 g for 5 min at 4 C
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This is especially relevant for smokers who constantly inhale cyanide through cigarettes
