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NMN treatment exerts beneficial effects on insulin secretion and insulin sensitivity in age- and diet-induced diabetes by restoring NAD + biosynthesis

A history of other substance use, higher socioeconomic status, or exposure to family violence or substance abuse has also been reported more often in this population (37, 38)

All analyses were performed in duplicate, and all analyses for each participant were performed within the same assay batch to reduce inter-assay variance in compliance with recommended laboratory procedures (21)

Ni-NTA resin (2 mL), pre-equilibrated with 20 mM imidazole (all buffer contained 150 mM NaCl and 20 mM HEPES pH 7.5), was added into the supernatant for batch binding with stirring at 4 C for 1.5 h