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Description
The development of early and late spherules has been described [4, 5]

look at the delivery method

Nat Protoc 1(2):581585 Couto N, Wood J, Barber J (2016) The role of glutathione reductase and related enzymes on cellular redox homoeostasis network

You can also add additional bacteriostatic water to bring the volume (and therefore the concentration) back to your intended ratio